The Subunit Structure of Yeast Pyruvate Kinase
نویسندگان
چکیده
منابع مشابه
Subunit structure of rabbit muscle pyruvate kinase.
Peptide mapping of rabbit muscle pyruvate kinase following tryptic digestion gave one-quarter the number of ninhydrin-reactive peptides, arginine-containing peptides, and tryptophan-containing peptides expected from the amino acid composition. Sedimentation velocity studies and disc gel electrophoresis of the enzyme subunits formed in 4 M urea and having one-quarter of the initial molecular wei...
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The kinetic properties of purified yeast pyruvate kinase were investigated. The enzyme showed cooperative kinetics toward the essential activating monovalent cations K+ and NH4+, Mg2+, and phosphoenolpyruvate. Fructose 1,6diphosphate, which yielded homotropic cooperative kinetics and did not affect maximal velocity, transformed the sigmoidal kinetics of Kf and NH4+, Mgz+, and phosphoenolpyruvat...
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The kinetics of pyruvate kinase from Saccharomyces cerevisiae were studied in assays at pH 6.2 where the relationships between the initial velocities of the catalysed reaction and the concentrations of the substrates ADP, phosphoenolpyruvate and Mg2+ are non-hyperbolic. The findings were represented empirically by the exponential model for a regulatory enzyme. The analysis shows that ADP, phosp...
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Background: The frequency of pyruvate kinase (PK) deficiency, an autosomal recessive defect, is approximately 3 per 10,000 individuals in Shiraz and surrounding areas, and is increased due to high consanguinity marriage frequency. The purpose of this study is to obtain data on the frequency and spectrum of gene mutation of PK in newborns, from Shiraz and surrounding areas. Materials and Methods...
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Hoggett, G. L. & Kellett, J. E. (1976) Bur. J. Biochem. 66,65-77 Lazarus, N. K., Derechin, M. & Barnard, E. A. (1968) Biochemistry 7, 2390-2400 McDonald, R. C., Steitz, T. A. & Engleman, D. M. (1979) Biochemistry 18,338-342 Menezes, L. C. & Pudles, J. (1977) Arch. Biochem. Biophys. 178, 34-42 Morris, H. R. & Dell, A. (1975) in Instrumentation in Amino Acid Sequence Analysis (Perham, R. N., ed.)...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1974
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1974.tb03660.x